Composite

Part:BBa_K3805542:Design

Designed by: Andi Zhai   Group: iGEM21_BNU-China   (2021-10-21)


mRNA switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 31


Design Notes

It is necessary to design DNA sequences with appropriate length to produce mRNA binding switches according to the types of different proteins. Taking proinsulin as an example, we hope to use this switch of mRNA interaction to ensure the normal growth of the strain without expressing the downstream killing system when the expression level of insulin mRNA is normal, and to start the expression and kill the cheaters when the mutation at the key site of proinsulin gene leads to the abnormal expression level of its mRNA. At the same time, we hope to explore the development possibility of mRNA interaction switch, and explore the optimal length ratio and optimal conditions of two mRNA interactions.

Source

No natural comparator

References